Hotspot

rpl2-trnI-CAU

LSC · 88500-89787 · 1288 bp

Back to Ceratostigma

Pi 0.0117

MarkerSeek score 63.60

Primer available yes

Label rpl2-trnI-CAU

Feature Metadata

label_name: rpl2-trnI-CAU
feature_type: igs
region: LSC
length_bp: 1288
variable_sites: 22
pi: 0.0117
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnI-CAU_p1	TTGTCCACCCTTTCCAAGTG	GCTAAGCATCCATGGCTGAA	50.0	50.0	57.9	58.3	1384-1424 bp	1.0	80.7
rpl2-trnI-CAU_p2	GCAATCAGTTTCGCTACAGC	GCTAAGCATCCATGGCTGAA	50.0	50.0	57.8	58.3	1422-1462 bp	1.0	79.8
rpl2-trnI-CAU_p3	ATCAGTTTCGCTACAGCACC	GCTAAGCATCCATGGCTGAA	50.0	50.0	58.3	58.3	1419-1459 bp	1.0	78.9
rpl2-trnI-CAU_p4	CCACCCTTTCCAAGTGTGAT	GCTAAGCATCCATGGCTGAA	50.0	50.0	57.7	58.3	1380-1420 bp	1.0	78.9
rpl2-trnI-CAU_p5	TTGTCCACCCTTTCCAAGTG	GCACCCAACTCATAATTGGC	50.0	50.0	57.9	57.4	1356-1396 bp	1.0	78.6