Hotspot

rpl33-rps18

LSC · 71998-72377 · 380 bp

Back to Ceratostigma

Pi 0.00798

MarkerSeek score 64.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 4
pi: 0.00798
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AAGAATCGACACAACACCCC	TACGAAAAGCGCGCTTAGAT	50.0	45.0	58.1	58.1	495-527 bp	1.0	83.1
rpl33-rps18_p2	AAGAATCGACACAACACCCC	TAAGGAGGGATCGCTTGGAT	50.0	50.0	58.1	57.9	474-506 bp	1.0	83.0
rpl33-rps18_p3	AAGAATCGACACAACACCCC	TTAAGGAGGGATCGCTTGGA	50.0	50.0	58.1	58.1	475-507 bp	1.0	82.8
rpl33-rps18_p4	AAGAATCGACACAACACCCC	CGAAAAGCGCGCTTAGATTT	50.0	45.0	58.1	57.8	493-525 bp	1.0	82.5
rpl33-rps18_p5	CCCCAGTCGGTTGGAATTAA	TACGAAAAGCGCGCTTAGAT	50.0	45.0	57.5	58.1	478-510 bp	1.0	80.0