Hotspot

ycf3-accD

LSC · 59709-60680 · 972 bp

Back to Ceratostigma

Pi 0.0695

MarkerSeek score 80.40

Primer available yes

Label ycf3-accD

Feature Metadata

label_name: ycf3-accD
feature_type: igs
region: LSC
length_bp: 972
variable_sites: 88
pi: 0.0695
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-accD_p1	TGAAATTGCAGAGGCTTGGT	GGGGAGTTGTTGGGACTTTT	45.0	50.0	58.0	57.9	1235-1299 bp	1.0	89.9
ycf3-accD_p2	TGAAACTCATGCACCTTGCT	GGGGAGTTGTTGGGACTTTT	45.0	50.0	58.0	57.9	1442-1502 bp	1.0	89.0
ycf3-accD_p3	TGAAATTGCAGAGGCTTGGT	GGGAATTGCTAGGGGAGTTG	45.0	55.0	58.0	57.9	1278-1310 bp	0.666667	75.8
ycf3-accD_p4	TGAAACTCATGCACCTTGCT	GGGAATTGCTAGGGGAGTTG	45.0	55.0	58.0	57.9	1485-1513 bp	0.666667	75.0
ycf3-accD_p5	GCTGAAGGTGGGACAAAAGA	GGGAATTGCTAGGGGAGTTG	50.0	55.0	58.0	57.9	1549-1577 bp	0.666667	74.8