Hotspot

trnS-GCU-trnG-UCC

LSC · 8513-9472 · 960 bp

Back to Ceratostigma

Pi 0.0163

MarkerSeek score 72.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 960
variable_sites: 22
pi: 0.0163
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	ATTGGGGATCTAACCGAGGC	50.0	55.0	58.2	59.2	1098-1135 bp	1.0	73.3
trnS-GCU-trnG-UCC_p2	CGAATCACTCGTACAACGGA	ATTGGGGATCTAACCGAGGC	50.0	55.0	57.7	59.2	1068-1105 bp	1.0	72.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AATTGGGGATCTAACCGAGG	50.0	50.0	58.2	56.7	1099-1136 bp	1.0	72.6
trnS-GCU-trnG-UCC_p4	CGAATCACTCGTACAACGGA	AATTGGGGATCTAACCGAGG	50.0	50.0	57.7	56.7	1069-1106 bp	1.0	71.9
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	AAATTGGGGATCTAACCGAGG	50.0	47.6	58.2	57.4	1100-1137 bp	1.0	70.6