Hotspot

rps16-trnQ-UUG

LSC · 6737-7329 · 593 bp

Back to Ceratostigma

Pi 0.00562

MarkerSeek score 63.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 5
pi: 0.00562
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCCACACCAGATCATGTCCT	TTTGGTCCCGCTATTCGAAG	50.0	50.0	58.0	58.0	705-1215 bp	1.0	80.2
rps16-trnQ-UUG_p2	TCCACACCAGATCATGTCCT	TACATTTTAGTGGGGCGTGG	50.0	50.0	58.0	57.9	746-1256 bp	1.0	79.4
rps16-trnQ-UUG_p3	TCCACACCAGATCATGTCCT	GTTTTGGTCCCGCTATTCGA	50.0	50.0	58.0	58.3	707-1217 bp	1.0	78.2
rps16-trnQ-UUG_p4	ACACACGTTGCTTTCTACCA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	57.7	58.0	680-1190 bp	1.0	78.0
rps16-trnQ-UUG_p5	ACACGTTGCTTTCTACCACA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	57.7	58.0	678-1188 bp	1.0	78.0