Hotspot

ycf1

SSC · 121126-126207 · 5082 bp

Back to Ceratopteris

Pi 0.221

MarkerSeek score 75.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5082
variable_sites: 1631
pi: 0.221
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGCTTCGAGAGATGTCACG	CTGATAGCGCCATAGTCGAC	50.0	55.0	58.0	58.0	835 bp	0.333333	53.0
ycf1_p2	TCGCCATTTCGCACAAAATC	CTGATAGCGCCATAGTCGAC	45.0	55.0	58.0	58.0	958 bp	0.333333	52.9
ycf1_p3	ACTTGCAATTTTCGTCGCAG	CTGATAGCGCCATAGTCGAC	45.0	55.0	58.0	58.0	530 bp	0.333333	52.8
ycf1_p4	CTTGCAATTTTCGTCGCAGT	CTGATAGCGCCATAGTCGAC	45.0	55.0	58.0	58.0	529 bp	0.333333	52.8
ycf1_p5	ATGTCACGCTTCAGCTGATT	CTGATAGCGCCATAGTCGAC	45.0	55.0	57.9	58.0	823 bp	0.333333	52.3