Hotspot

ndhA

SSC · 117264-119280 · 2017 bp

Back to Ceratopteris

Pi 0.163

MarkerSeek score 82.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2017
variable_sites: 466
pi: 0.163
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATCCAAAGTAACCGCGGATC	GTTGCTGCAGATCTTCCGTA	50.0	50.0	58.1	58.0	2956 bp	0.333333	52.8
ndhA_p2	GATCCAAAGTAACCGCGGAT	GTTGCTGCAGATCTTCCGTA	50.0	50.0	58.1	58.0	2957 bp	0.333333	52.8
ndhA_p3	TTTGCGGCTTCAACAACTTG	GTTGCTGCAGATCTTCCGTA	45.0	50.0	58.1	58.0	2922 bp	0.333333	52.6
ndhA_p4	ATCCAAAGTAACCGCGGATC	CCCCAACCCTTAAATTGCCT	50.0	50.0	58.1	58.0	2493 bp	0.333333	52.5
ndhA_p5	GATCCAAAGTAACCGCGGAT	CCCCAACCCTTAAATTGCCT	50.0	50.0	58.1	58.0	2494 bp	0.333333	52.5