Hotspot

clpP

LSC · 66057-67950 · 1894 bp

Back to Ceratopteris

Pi 0.154

MarkerSeek score 81.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1894
variable_sites: 420
pi: 0.154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CATGCCGGGAGTTAACGTTA	GTCCCCAATTCGGAGGTAAC	50.0	55.0	58.0	58.0	2649 bp	0.333333	53.0
clpP_p2	CAAAAAGATCGGGTTCGCAC	GGAAGCTCGCATCTTGAAGA	50.0	50.0	58.0	58.0	2949 bp	0.333333	52.9
clpP_p3	CAAAAAGATCGGGTTCGCAC	GTCCCCAATTCGGAGGTAAC	50.0	55.0	58.0	58.0	2502 bp	0.333333	52.8
clpP_p4	GGTATTTCATGCCGGGAGTT	GTCCCCAATTCGGAGGTAAC	50.0	55.0	57.9	58.0	2656 bp	0.333333	52.6
clpP_p5	CCTAGCAGTTGTCTCGCTTT	GGAAGCTCGCATCTTGAAGA	50.0	50.0	57.9	58.0	2713 bp	0.333333	52.5