Hotspot

trnI-CAU-trnT-UGU

IRb · 82530-84466 · 1937 bp

Back to Ceratopteris

Pi 0.209

MarkerSeek score 52.50

Primer available yes

Label trnI-CAU-trnT-UGU

Feature Metadata

label_name: trnI-CAU-trnT-UGU
feature_type: igs
region: IRb
length_bp: 1937
variable_sites: 142
pi: 0.209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.23
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-trnT-UGU_p1	TGAACCTGTGAATTCGCCAA	TTATACACGGCCTGACAACG	45.0	50.0	58.0	58.0	2204 bp	0.333333	53.1
trnI-CAU-trnT-UGU_p2	TTGAACCTGTGAATTCGCCA	TTATACACGGCCTGACAACG	45.0	50.0	58.0	58.0	2205 bp	0.333333	53.1
trnI-CAU-trnT-UGU_p3	ATGACTTGCATCTCGTTCCC	TTATACACGGCCTGACAACG	50.0	50.0	58.0	58.0	2836 bp	0.333333	53.1
trnI-CAU-trnT-UGU_p4	TGAACCTGTGAATTCGCCAA	AAAAGAAGGGGGTCGGGATA	45.0	50.0	58.0	58.0	2621 bp	0.333333	52.9
trnI-CAU-trnT-UGU_p5	TTGAACCTGTGAATTCGCCA	AAAAGAAGGGGGTCGGGATA	45.0	50.0	58.0	58.0	2622 bp	0.333333	52.9