Hotspot

clpP-psbB

LSC · 67951-68502 · 552 bp

Back to Ceratopteris

Pi 0.204

MarkerSeek score 80.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 552
variable_sites: 153
pi: 0.204
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	AAAGCCGCGAAAAATACAGC	50.0	45.0	58.0	58.0	1377 bp	0.333333	53.1
clpP-psbB_p2	CGCACTATACGTCAACCCAA	GGTCAAAGACAGCCAGTTCA	50.0	50.0	58.0	58.0	767 bp	0.333333	53.1
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGAACACTGAGGTGGAACAA	50.0	50.0	58.0	58.1	1287 bp	0.333333	52.9
clpP-psbB_p4	ACTCGCCCAAATGAGGAATC	AAAGCCGCGAAAAATACAGC	50.0	45.0	58.0	58.0	1934 bp	0.333333	52.8
clpP-psbB_p5	GCCCAAATGAGGAATCTCGT	AAAGCCGCGAAAAATACAGC	50.0	45.0	58.0	58.0	1930 bp	0.333333	52.8