Hotspot

petA-psbJ

LSC · 59438-60460 · 1023 bp

Back to Ceratopteris

Pi 0.271

MarkerSeek score 80.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 356
pi: 0.271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCTCAGCGACGGGAAAAATA	GAAGAGAAAGAGCGGGAAGG	50.0	55.0	58.0	58.0	1521 bp	0.333333	53.0
petA-psbJ_p2	CCTCAGCGACGGGAAAAATA	ACGACACAACCGAATCCAAA	50.0	45.0	58.0	58.0	1724 bp	0.333333	52.8
petA-psbJ_p3	AAGAGGGAAGAGGTGGGTAC	GAAGAGAAAGAGCGGGAAGG	55.0	55.0	58.0	58.0	1485 bp	0.333333	52.8
petA-psbJ_p4	TCCTATCACCAAACCCAGGT	GAAGAGAAAGAGCGGGAAGG	50.0	55.0	57.9	58.0	1650 bp	0.333333	52.8
petA-psbJ_p5	CCTCAGCGACGGGAAAAATA	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	1442 bp	0.333333	52.7