Hotspot

rpoB-trnD-GUC

LSC · 25981-26806 · 826 bp

Back to Ceratopteris

Pi 0.267

MarkerSeek score 81.90

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 289
pi: 0.267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	TCTCCGACTGAAGGTTCTGT	CATTGAGTAATCCAGCCCCC	50.0	55.0	58.0	58.0	1522 bp	0.333333	53.2
rpoB-trnD-GUC_p2	CTGGTACATATGGATCGGCG	CATTGAGTAATCCAGCCCCC	55.0	55.0	58.0	58.0	1575 bp	0.333333	53.2
rpoB-trnD-GUC_p3	CAAGTTCTGGTAGTACGGGC	CATTGAGTAATCCAGCCCCC	55.0	55.0	58.0	58.0	1371 bp	0.333333	53.2
rpoB-trnD-GUC_p4	GCAAGTTCTGGTAGTACGGG	CATTGAGTAATCCAGCCCCC	55.0	55.0	58.0	58.0	1372 bp	0.333333	53.2
rpoB-trnD-GUC_p5	GCATCCTCCGGTGATTCAAT	CATTGAGTAATCCAGCCCCC	50.0	55.0	58.0	58.0	1987 bp	0.333333	53.0