Hotspot

ccsA-ndhD

SSC · 117158-117345 · 188 bp

Back to Ceratophyllum

Pi 0.0488

MarkerSeek score 73.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 188
variable_sites: 16
pi: 0.0488
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGCGATTGGATCATTGGAGT	CAACTTCTTTCGGTCAACGC	50.0	50.0	58.0	58.0	1126-1129 bp	1.0	81.8
ccsA-ndhD_p2	ACCTGCTCTACAATCCCAGT	CAACTTCTTTCGGTCAACGC	50.0	50.0	58.0	58.0	1389-1392 bp	1.0	81.8
ccsA-ndhD_p3	GGCGATTGGATCATTGGAGT	CTCAATGGAGCCGTGTTACA	50.0	50.0	58.0	57.9	1022-1025 bp	1.0	81.5
ccsA-ndhD_p4	GGCGATTGGATCATTGGAGT	TGGTGCTGCACTCTTTTTCT	50.0	45.0	58.0	57.9	981-983 bp	0.75	70.2
ccsA-ndhD_p5	ACCTGCTCTACAATCCCAGT	TGGTGCTGCACTCTTTTTCT	50.0	45.0	58.0	57.9	1244-1246 bp	0.75	69.9