Hotspot

ycf1-ndhF

SSC · 111927-111951 · 25 bp

Back to Ceratophyllum

Pi 0.0933

MarkerSeek score 68.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 25
variable_sites: 4
pi: 0.0933
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CCCGGAACTTCTTCGTAGTG	TTGGTCATGTAATCGCGGTT	55.0	45.0	58.0	57.9	1011 bp	0.25	49.1
ycf1-ndhF_p2	CCGGAACTTCTTCGTAGTGG	TTGGTCATGTAATCGCGGTT	55.0	45.0	58.0	57.9	1010 bp	0.25	49.1
ycf1-ndhF_p3	GCTCGGGTTATGGAAGAAGG	TTGGTCATGTAATCGCGGTT	55.0	45.0	58.0	57.9	558 bp	0.25	48.8
ycf1-ndhF_p4	ACTCCCGGAACTTCTTCGTA	TTGGTCATGTAATCGCGGTT	50.0	45.0	58.1	57.9	1014 bp	0.25	48.5
ycf1-ndhF_p5	TCCCGGAACTTCTTCGTAGT	TTGGTCATGTAATCGCGGTT	50.0	45.0	58.1	57.9	1012 bp	0.25	48.5