Hotspot

petA-psbJ

LSC · 65510-66633 · 1124 bp

Back to Ceratophyllum

Pi 0.0176

MarkerSeek score 63.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1124
variable_sites: 39
pi: 0.0176
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTTCAAGGTCTCCTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1254-1261 bp	1.0	78.6
petA-psbJ_p2	ACGTGTTCAAGGTCTCCTGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.9	57.6	1255-1262 bp	1.0	73.5
petA-psbJ_p3	GGTCTCCTGTTCTTTTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.0	57.6	1245-1252 bp	1.0	72.8
petA-psbJ_p4	CGTGTTCAAGGTCTCCTGTT	GGTTCATATTCCGGATTGGGT	50.0	47.6	57.8	57.8	1261-1268 bp	1.0	71.7
petA-psbJ_p5	TACGTGTTCAAGGTCTCCTG	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.9	57.6	1256-1263 bp	1.0	71.7