Hotspot

ycf4-cemA

LSC · 62693-63634 · 942 bp

Back to Ceratophyllum

Pi 0.025

MarkerSeek score 65.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 942
variable_sites: 44
pi: 0.025
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AAAAAGCTGCTGAATTGGCC	ATGCAAGATATGGAAGGGGG	45.0	50.0	57.8	57.0	1032-1038 bp	1.0	75.0
ycf4-cemA_p2	AAAGCTGCTGAATTGGCCTA	ATGCAAGATATGGAAGGGGG	45.0	50.0	57.8	57.0	1030-1036 bp	1.0	74.6
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	GATGCAAGATATGGAAGGGGG	45.0	52.4	57.8	58.2	1033-1039 bp	1.0	73.3
ycf4-cemA_p4	AAAGCTGCTGAATTGGCCTA	GATGCAAGATATGGAAGGGGG	45.0	52.4	57.8	58.2	1031-1037 bp	1.0	72.9
ycf4-cemA_p5	AGCTGCTGAATTGGCCTATT	ATGCAAGATATGGAAGGGGG	45.0	50.0	57.6	57.0	1028-1034 bp	1.0	72.8