Hotspot

trnL-UAA-trnF-GAA

LSC · 49666-49980 · 315 bp

Back to Ceratophyllum

Pi 0.0364

MarkerSeek score 76.00

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 315
variable_sites: 22
pi: 0.0364
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	366-487 bp	1.0	83.6
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	402-523 bp	1.0	82.6
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	378-499 bp	1.0	82.4
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TTAACCACATGCCAGGAACC	50.0	50.0	57.5	58.1	425-546 bp	1.0	82.0
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	CACATGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	420-541 bp	1.0	81.5