Hotspot

ycf3-trnS-GGA

LSC · 45711-46528 · 818 bp

Back to Ceratophyllum

Pi 0.0166

MarkerSeek score 64.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 27
pi: 0.0166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	968-1012 bp	1.0	70.2
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	969-1013 bp	1.0	70.2
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	989-1033 bp	1.0	69.6
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	990-1034 bp	1.0	69.6
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	939-983 bp	1.0	69.5