Hotspot

trnE-UUC-trnT-GGU

LSC · 31864-32726 · 863 bp

Back to Ceratophyllum

Pi 0.0217
MarkerSeek score 69.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
863
variable_sites
36
pi
0.0217
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTAGTATCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.1 58.0 991-1004 bp 1.0 84.6
trnE-UUC-trnT-GGU_p2 CGTAGTATCCTACCCCCAGG CCATGGCGTTACTCTACCAC 60.0 55.0 58.1 58.1 970-983 bp 1.0 84.3
trnE-UUC-trnT-GGU_p3 CGTAGTATCCTACCCCCAGG TGGCGTTACTCTACCACTGA 60.0 50.0 58.1 58.1 967-980 bp 1.0 84.1
trnE-UUC-trnT-GGU_p4 CGTAGTATCCTACCCCCAGG CCGATGACTTACGCCTTACC 60.0 55.0 58.1 58.2 988-1001 bp 1.0 83.5
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 956-969 bp 1.0 83.3