Hotspot

petN-psbM

LSC · 28998-30027 · 1030 bp

Back to Ceratophyllum

Pi 0.0193

MarkerSeek score 66.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1030
variable_sites: 39
pi: 0.0193
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	ACTGCACTGTTCATTTTAGTTCC	50.0	39.1	57.6	57.6	1102-1127 bp	1.0	54.7
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	CTGCACTGTTCATTTTAGTTCCT	50.0	39.1	57.6	57.3	1101-1126 bp	1.0	52.6
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCACTGTTCATTTTAGTTCCT	50.0	36.4	57.6	56.2	1100-1125 bp	1.0	51.7
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	ACTGCACTGTTCATTTTAGTTCC	50.0	39.1	58.9	57.6	1155-1180 bp	1.0	50.0
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	CTGCACTGTTCATTTTAGTTCC	50.0	40.9	57.6	55.9	1101-1126 bp	1.0	49.5