Hotspot

rps16-trnQ-UUG

LSC · 6205-7018 · 814 bp

Back to Ceratophyllum

Pi 0.032
MarkerSeek score 67.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
814
variable_sites
51
pi
0.032
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 820-887 bp 1.0 87.0
rps16-trnQ-UUG_p2 CCTTCAATTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 830-897 bp 1.0 86.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 824-891 bp 1.0 85.9
rps16-trnQ-UUG_p4 CCTTCAATTCAAGTCGCACG CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.8 57.8 834-901 bp 1.0 85.1
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTGACATTTCAATGGGGCGT 45.0 45.0 58.1 58.4 880-947 bp 1.0 84.3