Hotspot

trnK-UUU-rps16

LSC · 4095-5031 · 937 bp

Back to Ceratophyllum

Pi 0.0269

MarkerSeek score 69.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 937
variable_sites: 50
pi: 0.0269
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGCATCAGGCACTAATCTT	GGCGACGAGACAAACAAAAG	50.0	50.0	58.0	58.0	1788-1886 bp	1.0	86.4
trnK-UUU-rps16_p2	CCGCATCAGGCACTAATCTT	AAAGGATCCCGAGACAAGGA	50.0	50.0	58.0	58.0	1866-1964 bp	1.0	86.2
trnK-UUU-rps16_p3	CCGCATCAGGCACTAATCTT	AAGGATCCCGAGACAAGGAA	50.0	50.0	58.0	58.0	1865-1963 bp	1.0	86.2
trnK-UUU-rps16_p4	CCGCATCAGGCACTAATCTT	AGGATCCCGAGACAAGGAAA	50.0	50.0	58.0	58.0	1864-1962 bp	1.0	86.2
trnK-UUU-rps16_p5	CCGCATCAGGCACTAATCTT	TCTTCAGTAAGCCACGGAGA	50.0	50.0	58.0	58.1	1670-1768 bp	1.0	86.0