Hotspot

rps19

LSC · 82330-82611 · 282 bp

Back to Ceratocephala

Pi 0.00709

MarkerSeek score 45.00

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 282
variable_sites: 2
pi: 0.00709
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TCGCTGTCTAGATAAGGCCA	ACTGATTGCAAAAGAGGGCA	50.0	45.0	57.9	58.0	1289 bp	1.0	79.7
rps19_p2	TCGCTGTCTAGATAAGGCCA	ATCCCAAAACTGCTCAGCAA	50.0	45.0	57.9	58.0	1226 bp	1.0	79.7
rps19_p3	TCGCTGTCTAGATAAGGCCA	TGGGTAGAGCCGGATCTAAG	50.0	55.0	57.9	58.0	1164 bp	1.0	79.7
rps19_p4	ATCTTCCTGTAGACCCTCGG	ACTGATTGCAAAAGAGGGCA	55.0	45.0	57.9	58.0	1403 bp	1.0	79.6
rps19_p5	ATCTTCCTGTAGACCCTCGG	ATCCCAAAACTGCTCAGCAA	55.0	45.0	57.9	58.0	1340 bp	1.0	79.6