Hotspot

ndhH

SSC · 119381-120562 · 1182 bp

Back to Ceratocephala

Pi 0.00169
MarkerSeek score 59.70
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
2
pi
0.00169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 GGAACAAGTAACCATATAAGCCC TGGTTTTAAGCAATCCGTGGA 43.5 42.9 57.0 58.1 1327 bp 1.0 40.1
ndhH_p2 GGAACAAGTAACCATATAAGCCC TGGTTTTAAGCAATCCGTGG 43.5 45.0 57.0 56.6 1327 bp 1.0 40.1
ndhH_p3 AGGAACAAGTAACCATATAAGCCC TGGTTTTAAGCAATCCGTGGA 41.7 42.9 58.3 58.1 1328 bp 1.0 40.1
ndhH_p4 GGAACAAGTAACCATATAAGCCC TTGGTTTTAAGCAATCCGTGG 43.5 42.9 57.0 57.3 1328 bp 1.0 40.1
ndhH_p5 GGAACAAGTAACCATATAAGCCCA TGGTTTTAAGCAATCCGTGGA 41.7 42.9 58.6 58.1 1327 bp 1.0 40.1