Hotspot

accD

LSC · 58198-59385 · 1188 bp

Back to Ceratocephala

Pi 0.00168
MarkerSeek score 59.70
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1188
variable_sites
2
pi
0.00168
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TCCCACGTATTCAGGAGGTT TCTTTTATCCTTACACTTCGAA 50.0 31.8 58.1 52.9 1279 bp 1.0 40.1
accD_p2 TCCCACGTATTCAGGAGGTT TTCTTTTATCCTTACACTTCGA 50.0 31.8 58.1 52.9 1280 bp 1.0 40.1
accD_p3 TTCCCACGTATTCAGGAGGT TCTTTTATCCTTACACTTCGAA 50.0 31.8 58.1 52.9 1280 bp 1.0 40.1
accD_p4 TTCCCACGTATTCAGGAGGT TTCTTTTATCCTTACACTTCGA 50.0 31.8 58.1 52.9 1281 bp 1.0 40.1
accD_p5 TCCCACGTATTCAGGAGGTT TTCTTTTATCCTTACACTTCGAA 50.0 30.4 58.1 53.7 1280 bp 1.0 40.1