Hotspot

rpl32-trnL-UAG

SSC · 111152-111757 · 606 bp

Back to Ceratocephala

Pi 0.00165
MarkerSeek score 59.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
606
variable_sites
1
pi
0.00165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAAAATCCCTTTCCACCGGA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 694 bp 1.0 79.4
rpl32-trnL-UAG_p2 CAAAATCCCTTTCCACCGGA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 695 bp 1.0 79.4
rpl32-trnL-UAG_p3 CAAAATCCCTTTCCACCGGA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 704 bp 1.0 78.5
rpl32-trnL-UAG_p4 CAAAATCCCTTTCCACCGGA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.1 57.8 703 bp 1.0 77.9
rpl32-trnL-UAG_p5 CAAAATCCCTTTCCACCGGA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.1 57.8 702 bp 1.0 77.9