Hotspot

trnF-GAA-ndhJ

LSC · 48463-49118 · 656 bp

Back to Ceratocephala

Pi 0.00152

MarkerSeek score 59.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 1
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	TAGTGCATCGGGAAAAGTCG	TTTGATGCCCGAAAGTTGGA	50.0	45.0	58.0	58.0	830 bp	1.0	80.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTTGATGCCCGAAAGTTGGA	50.0	45.0	58.1	58.0	775 bp	1.0	79.6
trnF-GAA-ndhJ_p3	TAGTGCATCGGGAAAAGTCG	TCCACGCCTGAAACGTATTT	50.0	45.0	58.0	57.8	848 bp	1.0	78.7
trnF-GAA-ndhJ_p4	TAGTGCATCGGGAAAAGTCG	TTGATGCCCGAAAGTTGGAT	50.0	45.0	58.0	57.8	829 bp	1.0	78.3
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	50.0	45.0	58.1	57.8	793 bp	1.0	78.3