Hotspot

trnS-GGA-rps4

LSC · 45293-46056 · 764 bp

Back to Ceratocephala

Pi 0.00262

MarkerSeek score 54.10

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 2
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TGTGGGCTTTTGTTTACCGA	CTAGTAAAAGGCCGACACCC	45.0	55.0	57.9	58.0	896 bp	1.0	79.6
trnS-GGA-rps4_p2	GGAACTGCTATGTGGGCTTT	CTAGTAAAAGGCCGACACCC	50.0	55.0	58.2	58.0	906 bp	1.0	78.7
trnS-GGA-rps4_p3	TGTGGGCTTTTGTTTACCGA	CCAACAGCTCTACGGGAAAA	45.0	50.0	57.9	57.8	859 bp	1.0	78.3
trnS-GGA-rps4_p4	ATTGGAACTGCTATGTGGGC	CTAGTAAAAGGCCGACACCC	50.0	55.0	58.2	58.0	909 bp	1.0	78.2
trnS-GGA-rps4_p5	ACTGCTATGTGGGCTTTTGT	CTAGTAAAAGGCCGACACCC	45.0	55.0	57.7	58.0	903 bp	1.0	77.7