Hotspot

trnT-GGU-psbD

LSC · 31431-32821 · 1391 bp

Back to Ceratocephala

Pi 0.00144

MarkerSeek score 59.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1391
variable_sites: 2
pi: 0.00144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCGTAACCAGTCATCCATCG	55.0	55.0	58.1	58.1	1521 bp	1.0	78.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCGTAACCAGTCATCCATCG	50.0	55.0	58.1	58.1	1524 bp	1.0	78.2
trnT-GGU-psbD_p3	TTGGTTCATTCAGGAGTGGC	CCGTAACCAGTCATCCATCG	50.0	55.0	58.1	58.1	1564 bp	1.0	78.2
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TCTCCGTAACCAGTCATCCA	55.0	50.0	58.1	57.8	1524 bp	1.0	77.8
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.1	57.8	1527 bp	1.0	77.6