Hotspot

psbM-trnD-GUC

LSC · 29480-29878 · 399 bp

Back to Ceratocephala

Pi 0.00251

MarkerSeek score 56.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 1
pi: 0.00251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTGACTGACCGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.0	57.9	561 bp	1.0	79.4
psbM-trnD-GUC_p2	CAGCGCAGTAGCAATGAATG	AGTTCAATCGGTCAGAGCAC	50.0	50.0	57.9	57.9	504 bp	1.0	78.7
psbM-trnD-GUC_p3	ACAGCGCAGTAGCAATGAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	505 bp	1.0	77.4
psbM-trnD-GUC_p4	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	509 bp	1.0	77.4
psbM-trnD-GUC_p5	AACAGCGCAGTAGCAATGAA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	506 bp	1.0	75.7