Hotspot

rpoC1

LSC · 20954-23765 · 2812 bp

Back to Cerasus

Pi 0.000711

MarkerSeek score 56.40

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2812
variable_sites: 2
pi: 0.000711
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	ACTCACACGGGTGTCATAGA	TTTGGCTCTGGAACTGAACC	50.0	50.0	58.1	58.0	2958 bp	1.0	79.3
rpoC1_p2	ACTCACACGGGTGTCATAGA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	58.1	57.9	2964 bp	1.0	78.6
rpoC1_p3	ACTCACACGGGTGTCATAGA	GGCTCTGGAACTGAACCATT	50.0	50.0	58.1	57.8	2955 bp	1.0	77.8
rpoC1_p4	CAAACTCACACGGGTGTCAT	TTTGGCTCTGGAACTGAACC	50.0	50.0	58.4	58.0	2961 bp	1.0	76.6
rpoC1_p5	ACTCACACGGGTGTCATAGA	CGATCTTTGGCTCTGGAACT	50.0	50.0	58.1	57.6	2963 bp	1.0	76.3