Hotspot

rpoB

LSC · 23797-27009 · 3213 bp

Back to Cerasus

Pi 0.000934

MarkerSeek score 52.20

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 3
pi: 0.000934
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TGTGTCAATGGATTTGTTCGA	CGCGAGATATATTGGCAGCT	38.1	50.0	56.1	58.0	310 bp	1.0	57.3
rpoB_p2	TGTCAATGGATTTGTTCGATCC	CGCGAGATATATTGGCAGCT	40.9	50.0	57.0	58.0	308 bp	1.0	56.8
rpoB_p3	TGTGTCAATGGATTTGTTCGA	GATATATTGGCAGCTGCGGA	38.1	50.0	56.1	57.9	305 bp	1.0	56.5
rpoB_p4	TGTGTCAATGGATTTGTTCGA	TATATTGGCAGCTGCGGATC	38.1	50.0	56.1	57.9	303 bp	1.0	56.5
rpoB_p5	TGTGTCAATGGATTTGTTCGA	AGATATATTGGCAGCTGCGG	38.1	50.0	56.1	57.9	306 bp	1.0	56.5