Hotspot

rbcL

LSC · 56815-58242 · 1428 bp

Back to Cerasus

Pi 0.000713

MarkerSeek score 60.90

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 1
pi: 0.000713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	AGTAAAAGATTGGGCCGAGT	50.0	45.0	57.3	56.8	1539-1564 bp	1.0	66.2
rbcL_p2	TGATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGT	50.0	45.0	57.1	56.8	1542-1567 bp	1.0	64.4
rbcL_p3	TGAGTTGTAGGGAGGGACTT	AGTAAAAGATTGGGCCGAGTT	50.0	42.9	57.3	57.6	1539-1564 bp	1.0	64.1
rbcL_p4	TTGATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGT	47.6	45.0	57.8	56.8	1543-1568 bp	1.0	62.3
rbcL_p5	TGATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGTT	50.0	42.9	57.1	57.6	1542-1567 bp	1.0	62.3