Hotspot

clpP

LSC · 71711-73779 · 2069 bp

Back to Cerasus

Pi 0.0029

MarkerSeek score 59.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2069
variable_sites: 6
pi: 0.0029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGAATTCGTGTGAAATCCTTGT	CCCATTGTTACGCTTTCACA	40.9	45.0	57.6	56.6	2161 bp	1.0	50.1
clpP_p2	CGAATTCGTGTGAAATCCTTGT	ACCCATTGTTACGCTTTCAC	40.9	45.0	57.6	56.6	2162 bp	1.0	50.1
clpP_p3	CGAATTCGTGTGAAATCCTTGT	ACCCATTGTTACGCTTTCACA	40.9	42.9	57.6	58.4	2162 bp	1.0	49.6
clpP_p4	CGAATTCGTGTGAAATCCTTGT	AACCCATTGTTACGCTTTCAC	40.9	42.9	57.6	57.4	2163 bp	1.0	47.9
clpP_p5	CGAATTCGTGTGAAATCCTTGT	CCCATTGTTACGCTTTCACAT	40.9	42.9	57.6	57.2	2161 bp	1.0	46.2