Hotspot

cemA

LSC · 62766-63455 · 690 bp

Back to Cerasus

Pi 0.0029

MarkerSeek score 60.40

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 2
pi: 0.0029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TCGTATAGAGTCGACGAACG	AGGAATCCCTTGGATGGGTA	50.0	50.0	56.7	57.4	849 bp	1.0	65.2
cemA_p2	CGAGGTGCGTTCAGTAAAAA	AGGAATCCCTTGGATGGGTA	45.0	50.0	56.7	57.4	831 bp	1.0	64.9
cemA_p3	TCATGGGCTCGATAACTTGT	AGGAATCCCTTGGATGGGTA	45.0	50.0	56.6	57.4	901 bp	1.0	64.4
cemA_p4	TCGTATAGAGTCGACGAACG	GGAATCCCTTGGATGGGTAG	50.0	55.0	56.7	57.0	848 bp	1.0	62.4
cemA_p5	TTCATGGGCTCGATAACTTGT	AGGAATCCCTTGGATGGGTA	42.9	50.0	57.4	57.4	902 bp	1.0	62.3