Hotspot

ndhB-trnL-CAA

IRa · 147041-147659 · 619 bp

Back to Cerasus

Pi 0.00162
MarkerSeek score 59.70
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
IRa
length_bp
619
variable_sites
1
pi
0.00162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 CCAGGAATATCAATGAACCCGA GTCTCGCGTGTCTACCATTT 45.5 50.0 57.9 58.0 705 bp 1.0 63.4
ndhB-trnL-CAA_p2 CCAGGAATATCAATGAACCCGA CTCGCGTGTCTACCATTTCA 45.5 50.0 57.9 58.0 703 bp 1.0 63.4
ndhB-trnL-CAA_p3 CCAGGAATATCAATGAACCCGA TACCATTTCACCACCAAGGC 45.5 50.0 57.9 58.1 693 bp 1.0 62.8
ndhB-trnL-CAA_p4 ACCAGGAATATCAATGAACCCG GTCTCGCGTGTCTACCATTT 45.5 50.0 58.2 58.0 706 bp 1.0 62.6
ndhB-trnL-CAA_p5 ACCAGGAATATCAATGAACCCG CTCGCGTGTCTACCATTTCA 45.5 50.0 58.2 58.0 704 bp 1.0 62.6