Hotspot

rpl32-trnL-UAG

SSC · 115711-117103 · 1393 bp

Back to Cerasus

Pi 0.00226

MarkerSeek score 64.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1393
variable_sites: 3
pi: 0.00226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGATATCGGGCAGCATTGAA	AGAGCAGCGTGTCTACCTAT	50.0	50.0	58.1	57.9	1506-1517 bp	1.0	82.2
rpl32-trnL-UAG_p2	GGATATCGGGCAGCATTGAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1511-1522 bp	1.0	81.7
rpl32-trnL-UAG_p3	GGGCAGCATTGAAAGCTTTT	AGAGCAGCGTGTCTACCTAT	45.0	50.0	57.8	57.9	1499-1510 bp	1.0	81.6
rpl32-trnL-UAG_p4	GGATATCGGGCAGCATTGAA	AAGAGCAGCGTGTCTACCTA	50.0	50.0	58.1	58.2	1507-1518 bp	1.0	81.3
rpl32-trnL-UAG_p5	GGATATCGGGCAGCATTGAA	TAAGAGCAGCGTGTCTACCT	50.0	50.0	58.1	58.2	1508-1519 bp	1.0	81.3