Hotspot

psbE-petL

LSC · 66382-67660 · 1279 bp

Back to Cerasus

Pi 0.00313

MarkerSeek score 60.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1279
variable_sites: 4
pi: 0.00313
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1394-1395 bp	1.0	77.6
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.2	58.2	1480-1481 bp	1.0	77.5
psbE-petL_p3	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCG	50.0	55.0	58.2	58.2	1485-1486 bp	1.0	77.4
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCG	55.0	55.0	58.2	58.2	1389-1390 bp	1.0	77.3
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.8	58.3	1393-1394 bp	1.0	77.0