Hotspot

accD-psaI

LSC · 60361-61134 · 774 bp

Back to Cerasus

Pi 0.0013
MarkerSeek score 60.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
774
variable_sites
1
pi
0.0013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CTTTAAAGGGCGTTCTGGGT CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 906-912 bp 1.0 79.6
accD-psaI_p2 GGCGTTCTGGGTGAGTTATT CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 898-904 bp 1.0 79.5
accD-psaI_p3 CTTTAAAGGGCGTTCTGGGT TTGCCGGAAATACTAAGCCC 50.0 50.0 58.1 58.0 897-903 bp 1.0 79.5
accD-psaI_p4 GGCGTTCTGGGTGAGTTATT TTGCCGGAAATACTAAGCCC 50.0 50.0 57.9 58.0 889-895 bp 1.0 79.4
accD-psaI_p5 CTTTAAAGGGCGTTCTGGGT AAGAAGCCATTGCAATTGCC 50.0 45.0 58.1 57.9 912-918 bp 1.0 79.0