Hotspot

atpB-rbcL

LSC · 56073-56814 · 742 bp

Back to Cerasus

Pi 0.0027

MarkerSeek score 60.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 2
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	AACACCAGCTTTGAACCCAA	55.0	45.0	58.0	58.1	890 bp	1.0	78.7
atpB-rbcL_p2	CGGTGGAAAAGCTACATCCA	AACACCAGCTTTGAACCCAA	50.0	45.0	57.9	58.1	904 bp	1.0	78.2
atpB-rbcL_p3	ATCCAGTACCGGACCAATGA	AACACCAGCTTTGAACCCAA	50.0	45.0	58.1	58.1	889 bp	1.0	78.0
atpB-rbcL_p4	TCCAGTACCGGACCAATGAT	AACACCAGCTTTGAACCCAA	50.0	45.0	58.1	58.1	888 bp	1.0	78.0
atpB-rbcL_p5	AAGCTACATCCAGTACCGGA	AACACCAGCTTTGAACCCAA	50.0	45.0	57.8	58.1	896 bp	1.0	77.8