Hotspot

rpoB-trnC-GCA

LSC · 27010-28189 · 1180 bp

Back to Cerasus

Pi 0.00169

MarkerSeek score 59.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1180
variable_sites: 2
pi: 0.00169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TAAGCCCTGCTCAATGAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1335 bp	1.0	78.4
rpoB-trnC-GCA_p2	ATTGCCTCATTTACACCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1268 bp	1.0	78.2
rpoB-trnC-GCA_p3	CTCATTTACACCCCCAAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1263 bp	1.0	77.8
rpoB-trnC-GCA_p4	TAAGCCCTGCTCAATGAACC	TCTTTTGGTGATCAGGCGAC	50.0	50.0	57.9	58.2	1357 bp	1.0	77.6
rpoB-trnC-GCA_p5	ATTGCCTCATTTACACCCCC	TCTTTTGGTGATCAGGCGAC	50.0	50.0	57.8	58.2	1290 bp	1.0	77.3