Hotspot

petG-trnW-CCA

LSC · 118328-118449 · 122 bp

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Pi 0.0219

MarkerSeek score 59.80

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 8
pi: 0.0219
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CGTGGTGATCAGTTGGACTT	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	177-192 bp	1.0	85.3
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	182-197 bp	1.0	85.2
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	218-233 bp	1.0	84.6
petG-trnW-CCA_p4	CGTGGTGATCAGTTGGACTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	213-228 bp	1.0	84.6
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	216-231 bp	1.0	82.0