Hotspot

trnT-UGU-trnL-UAA

LSC · 99180-99828 · 649 bp

Back to Cerastium

Pi 0.0159

MarkerSeek score 70.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 24
pi: 0.0159
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	555-710 bp	1.0	83.9
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	587-742 bp	1.0	82.9
trnT-UGU-trnL-UAA_p3	AAAGCCCACTATCGGAATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	600-755 bp	1.0	82.8
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	549-704 bp	1.0	82.6
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	581-736 bp	1.0	81.6