Hotspot

rps4-trnT-UGU

LSC · 98755-99106 · 352 bp

Back to Cerastium

Pi 0.00947

MarkerSeek score 46.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 10
pi: 0.00947
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GAACGGGATTGGGCTCTAAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.0	57.9	528-532 bp	1.0	80.7
rps4-trnT-UGU_p2	GAACGGGATTGGGCTCTAAG	GGTTCGATTCCGATAGTGGG	55.0	55.0	58.0	57.8	487-491 bp	1.0	80.7
rps4-trnT-UGU_p3	GCTCTAAGATCACTTCCGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	516-520 bp	1.0	80.0
rps4-trnT-UGU_p4	GAACGGGATTGGGCTCTAAG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.0	57.8	496-500 bp	1.0	80.0
rps4-trnT-UGU_p5	GAACGGGATTGGGCTCTAAG	TTTCTATGAGCCCGCTTAGC	55.0	50.0	58.0	57.8	547-551 bp	1.0	79.8