Hotspot

psaA-ycf3

LSC · 94730-95556 · 827 bp

Back to Cerastium

Pi 0.014

MarkerSeek score 62.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 27
pi: 0.014
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	TTGGTTGAAGATCACGGGAC	61.1	50.0	58.4	57.8	856-1026 bp	1.0	65.3
psaA-ycf3_p2	ACTTCTGGTTCCGGCGAA	TTGGTTGAAGATCACGGGAC	55.6	50.0	58.9	57.8	857-1027 bp	1.0	62.0
psaA-ycf3_p3	ACTTCTGGTTCCGGCGAAC	TTGGTTGAAGATCACGGGAC	57.9	50.0	60.3	57.8	857-1027 bp	1.0	58.4
psaA-ycf3_p4	CTTCTGGTTCCGGCGAAC	ATTGGTTGAAGATCACGGGA	61.1	45.0	58.4	56.8	857-1027 bp	1.0	57.4
psaA-ycf3_p5	CTTCTGGTTCCGGCGAAC	ATTGGTTGAAGATCACGGGAC	61.1	47.6	58.4	58.3	857-1027 bp	1.0	56.5