Hotspot

trnT-GGU-psbD

LSC · 84828-85909 · 1082 bp

Back to Cerastium

Pi 0.00961

MarkerSeek score 59.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1082
variable_sites: 29
pi: 0.00961
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TGGTAAATTGACCAACAGCT	55.0	40.0	58.1	55.2	1156-1194 bp	1.0	61.1
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TGGTAAATTGACCAACAGCT	50.0	40.0	58.1	55.2	1159-1197 bp	1.0	60.9
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TGGTAAATTGACCAACAGCT	50.0	40.0	58.3	55.2	1133-1171 bp	1.0	59.4
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	TGGTAAATTGACCAACAGCT	50.0	40.0	57.7	55.2	1171-1209 bp	1.0	59.2
trnT-GGU-psbD_p5	TGGTAGAGTAACGCCATGGT	TGGTAAATTGACCAACAGCT	50.0	40.0	58.4	55.2	1155-1193 bp	1.0	58.1