Hotspot

psbI-trnS-GCU

LSC · 63063-63240 · 178 bp

Back to Cerastium

Pi 0.0187

MarkerSeek score 58.10

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 10
pi: 0.0187
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	AAGAGGGAGAGATGGCTGAG	50.0	55.0	58.3	58.2	305-317 bp	1.0	79.1
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	TTCAAGAGGGAGAGATGGCT	50.0	50.0	58.3	57.7	308-320 bp	1.0	78.6
psbI-trnS-GCU_p3	ATGATCCAGGGCGTAATCCT	CGAGTTATTCGTACCGAGGG	50.0	55.0	58.3	57.6	252-264 bp	1.0	78.2
psbI-trnS-GCU_p4	ATGATCCAGGGCGTAATCCT	CAAGAGGGAGAGATGGCTGA	50.0	55.0	58.3	58.5	306-318 bp	1.0	76.7
psbI-trnS-GCU_p5	ATGATCCAGGGCGTAATCCT	TCAAGAGGGAGAGATGGCTG	50.0	55.0	58.3	58.5	307-319 bp	1.0	76.7