Hotspot

trnS-GCU

Genome · 27821-27908 · 88 bp

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Pi 0.0107

MarkerSeek score 47.00

Primer available yes

Label trnS-GCU

Feature Metadata

label_name: trnS-GCU
feature_type: tRNA
region: Genome
length_bp: 88
variable_sites: 3
pi: 0.0107
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU_p1	CTGGGCGTAAAGAACAGTGA	TCGCAAATCGCAAATGTTGG	50.0	45.0	57.8	58.3	191 bp	1.0	77.5
trnS-GCU_p2	CTGGGCGTAAAGAACAGTGA	GGATTCTCGCAAATCGCAAA	50.0	45.0	57.8	57.5	197 bp	1.0	75.6
trnS-GCU_p3	ATCCTGGGCGTAAAGAACAG	TCGCAAATCGCAAATGTTGG	50.0	45.0	57.6	58.3	194 bp	1.0	75.6
trnS-GCU_p4	CTGGGCGTAAAGAACAGTGA	TGGATTCTCGCAAATCGCAA	50.0	45.0	57.8	58.6	198 bp	1.0	75.5
trnS-GCU_p5	CTGGGCGTAAAGAACAGTGA	TTGGATTCTCGCAAATCGCA	50.0	45.0	57.8	58.6	199 bp	1.0	75.5