Hotspot

accD

Genome · 19779-22937 · 3159 bp

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Pi 0.00827

MarkerSeek score 47.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 3159
variable_sites: 71
pi: 0.00827
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTGACCGATGCCTGTTTGAA	TGCAGCACGTCTAAAAGAGA	45.0	45.0	58.0	57.2	802-1018 bp	1.0	82.5
accD_p2	ACCTACTACCGAGCCCATAC	TGCAGCACGTCTAAAAGAGA	55.0	45.0	58.0	57.2	877-1093 bp	1.0	82.3
accD_p3	CACCTACTACCGAGCCCATA	TGCAGCACGTCTAAAAGAGA	55.0	45.0	58.0	57.2	878-1094 bp	1.0	82.3
accD_p4	GCCAGAAGCACATACGAGAA	TGCAGCACGTCTAAAAGAGA	50.0	45.0	58.0	57.2	949-1165 bp	1.0	82.0
accD_p5	ACCTACTACCGAGCCCATAC	ACTTTTTGGGTGGACTTCCA	55.0	45.0	58.0	57.2	911-1127 bp	1.0	82.0