Hotspot

rrn16-rps12

Genome · 114731-116296 · 1566 bp

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Pi 0.00584

MarkerSeek score 53.00

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: Genome
length_bp: 1566
variable_sites: 26
pi: 0.00584
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	ACTTCCCGTTTGACTTGCAT	TGGCAACTTTACGTAAGGCA	45.0	45.0	58.0	57.7	1687-1758 bp	1.0	79.0
rrn16-rps12_p2	AAACACCACTTCCCGTTTGA	TGGCAACTTTACGTAAGGCA	45.0	45.0	57.9	57.7	1694-1765 bp	1.0	78.1
rrn16-rps12_p3	ACTTCCCGTTTGACTTGCAT	AATCGCACTCTGGCAACTTT	45.0	45.0	58.0	58.4	1697-1768 bp	1.0	78.0
rrn16-rps12_p4	ACTTCCCGTTTGACTTGCAT	ATCGCACTCTGGCAACTTTA	45.0	45.0	58.0	57.5	1696-1767 bp	1.0	77.3
rrn16-rps12_p5	ACTTCCCGTTTGACTTGCAT	TAATCGCACTCTGGCAACTT	45.0	45.0	58.0	57.5	1698-1769 bp	1.0	77.3